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cy5 5  (MedChemExpress)
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MedChemExpress cy5 5
Preparation and characterization of NM@MRP-NP ( A ) Morphology of MRP-NP, NM and NM@MRP-NP observed by SEM. Scale bar = 100 nm. ( B - D ) Size distributions ( B ), Z-average diameter ( C ) and zeta potential ( D ) of MRP-NP, NM and NM@MRP-NP measured by DLS ( n = 5). ( E ) Z-average diameter of MRP-NP, NM and NM@MRP-NP after incubation with mouse serum or FBS for different durations ( n = 5). ( F ) Fluorescence image of NM@MRP-NP loaded with different concentrations of <t>Cy5.5.</t> ( G ) Drug release rates of MRP-NP and NM@MRP-NP with or without MMP2 ( n = 5)
Cy5 5, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Preparation and characterization of NM@MRP-NP ( A ) Morphology of MRP-NP, NM and NM@MRP-NP observed by SEM. Scale bar = 100 nm. ( B - D ) Size distributions ( B ), Z-average diameter ( C ) and zeta potential ( D ) of MRP-NP, NM and NM@MRP-NP measured by DLS ( n = 5). ( E ) Z-average diameter of MRP-NP, NM and NM@MRP-NP after incubation with mouse serum or FBS for different durations ( n = 5). ( F ) Fluorescence image of NM@MRP-NP loaded with different concentrations of Cy5.5. ( G ) Drug release rates of MRP-NP and NM@MRP-NP with or without MMP2 ( n = 5)

Journal: Journal of Nanobiotechnology

Article Title: Neutrophil membrane-coated and MMP2-responsive nanoparticles deliver PIM1 inhibitor to alleviate inflammatory arthritis through inhibiting Th17 cell differentiation

doi: 10.1186/s12951-026-04280-x

Figure Lengend Snippet: Preparation and characterization of NM@MRP-NP ( A ) Morphology of MRP-NP, NM and NM@MRP-NP observed by SEM. Scale bar = 100 nm. ( B - D ) Size distributions ( B ), Z-average diameter ( C ) and zeta potential ( D ) of MRP-NP, NM and NM@MRP-NP measured by DLS ( n = 5). ( E ) Z-average diameter of MRP-NP, NM and NM@MRP-NP after incubation with mouse serum or FBS for different durations ( n = 5). ( F ) Fluorescence image of NM@MRP-NP loaded with different concentrations of Cy5.5. ( G ) Drug release rates of MRP-NP and NM@MRP-NP with or without MMP2 ( n = 5)

Article Snippet: The resulting NM@MRP-NP was collected by centrifugation at 20,000×g at 4 °C for 20 min. For the fluorescence tracing experiment, Cy5.5 (MCE, #HY-D0924) was substituted for AZD1208 and encapsulated into the nanoparticles.

Techniques: Zeta Potential Analyzer, Incubation, Fluorescence

Biological characteristics of NM@MRP-NP. ( A ) Western blot analysis of the expression of CD11a, CD11b and L-selectin on the neutrophil membrane before and after LPS treatment ( n = 9). ( B ) Representative immunofluorescence images of CD11a, CD11b and L-selectin on the neutrophil membrane before and after LPS treatment. Scale bar = 20 μm. ( C - D ) Western blot analysis ( C ) and immunofluorescence staining ( D ) confirmed the transfer of CD11a, CD11b and L-selectin to the NM@MRP-NP. Scale bar = 100 μm. ( E - F ) Uptake of Cy5.5 by activated or unactivated CD4 + T cells treated with vehicle, MRP-NP, NM@MRP-NP or NM@MRP-NP+MMP2 ( n = 9). The statistical analyses were performed as follows: paired t test (A, F) and one-way ANOVA followed by Bonferroni’s post hoc comparisons test ( F ). ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001, and n.s. indicates no statistical significance

Journal: Journal of Nanobiotechnology

Article Title: Neutrophil membrane-coated and MMP2-responsive nanoparticles deliver PIM1 inhibitor to alleviate inflammatory arthritis through inhibiting Th17 cell differentiation

doi: 10.1186/s12951-026-04280-x

Figure Lengend Snippet: Biological characteristics of NM@MRP-NP. ( A ) Western blot analysis of the expression of CD11a, CD11b and L-selectin on the neutrophil membrane before and after LPS treatment ( n = 9). ( B ) Representative immunofluorescence images of CD11a, CD11b and L-selectin on the neutrophil membrane before and after LPS treatment. Scale bar = 20 μm. ( C - D ) Western blot analysis ( C ) and immunofluorescence staining ( D ) confirmed the transfer of CD11a, CD11b and L-selectin to the NM@MRP-NP. Scale bar = 100 μm. ( E - F ) Uptake of Cy5.5 by activated or unactivated CD4 + T cells treated with vehicle, MRP-NP, NM@MRP-NP or NM@MRP-NP+MMP2 ( n = 9). The statistical analyses were performed as follows: paired t test (A, F) and one-way ANOVA followed by Bonferroni’s post hoc comparisons test ( F ). ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001, and n.s. indicates no statistical significance

Article Snippet: The resulting NM@MRP-NP was collected by centrifugation at 20,000×g at 4 °C for 20 min. For the fluorescence tracing experiment, Cy5.5 (MCE, #HY-D0924) was substituted for AZD1208 and encapsulated into the nanoparticles.

Techniques: Western Blot, Expressing, Membrane, Immunofluorescence, Staining

Targeting effect of NM@MRP-NP in vivo. ( A ) Cy5.5 fluorescence images of the inflamed ankles of SKG mice at different time points after treatment with vehicle, free Cy5.5, Cy5.5-labeled MRP-NP or NM@MRP-NP. ( B ) Cy5.5 fluorescence images of synovial fluid collected from SKG mice 8 h after treatment with vehicle, free Cy5.5, Cy5.5-labeled MRP-NP or NM@MRP-NP. ( C ) Cy5.5 fluorescence images of the ankles and the major organs dissected from SKG mice 8 h after treatment with vehicle, free Cy5.5, Cy5.5-labeled MRP-NP or NM@MRP-NP. ( D ) Immunofluorescence images of Cy5.5 and CD4 + T cells in the inflamed joints of SKG mice treated with vehicle, free Cy5.5, Cy5.5-labeled MRP-NP or NM@MRP-NP. Scale bar = 50 μm. ( E ) Quantitative analysis of the Cy5.5 fluorescent signal in the inflamed ankles of SKG mice at different time points after receiving Cy5.5-labeled NM@MRP-NP ( n = 6). ( F ) Quantitative analysis of Cy5.5 fluorescent signal in synovial fluid collected from SKG mice 8 h after treatment with vehicle, free Cy5.5, Cy5.5-labeled MRP-NP or NM@MRP-NP ( n = 5). ( G ) Quantitative analysis of Cy5.5 fluorescence signal in the ankles and the major organs dissected from SKG mice 8 h after treatment with vehicle, free Cy5.5, Cy5.5-labeled MRP-NP or NM@MRP-NP ( n = 5). ( H ) Line-scan analysis of the colocalization of Cy5.5 and CD4 + T cells in the immunofluorescence images in Fig. 7D. The statistical analyses were performed as follows: one-way ANOVA followed by Bonferroni’s post hoc comparisons test (F, G). **** indicates p < 0.0001

Journal: Journal of Nanobiotechnology

Article Title: Neutrophil membrane-coated and MMP2-responsive nanoparticles deliver PIM1 inhibitor to alleviate inflammatory arthritis through inhibiting Th17 cell differentiation

doi: 10.1186/s12951-026-04280-x

Figure Lengend Snippet: Targeting effect of NM@MRP-NP in vivo. ( A ) Cy5.5 fluorescence images of the inflamed ankles of SKG mice at different time points after treatment with vehicle, free Cy5.5, Cy5.5-labeled MRP-NP or NM@MRP-NP. ( B ) Cy5.5 fluorescence images of synovial fluid collected from SKG mice 8 h after treatment with vehicle, free Cy5.5, Cy5.5-labeled MRP-NP or NM@MRP-NP. ( C ) Cy5.5 fluorescence images of the ankles and the major organs dissected from SKG mice 8 h after treatment with vehicle, free Cy5.5, Cy5.5-labeled MRP-NP or NM@MRP-NP. ( D ) Immunofluorescence images of Cy5.5 and CD4 + T cells in the inflamed joints of SKG mice treated with vehicle, free Cy5.5, Cy5.5-labeled MRP-NP or NM@MRP-NP. Scale bar = 50 μm. ( E ) Quantitative analysis of the Cy5.5 fluorescent signal in the inflamed ankles of SKG mice at different time points after receiving Cy5.5-labeled NM@MRP-NP ( n = 6). ( F ) Quantitative analysis of Cy5.5 fluorescent signal in synovial fluid collected from SKG mice 8 h after treatment with vehicle, free Cy5.5, Cy5.5-labeled MRP-NP or NM@MRP-NP ( n = 5). ( G ) Quantitative analysis of Cy5.5 fluorescence signal in the ankles and the major organs dissected from SKG mice 8 h after treatment with vehicle, free Cy5.5, Cy5.5-labeled MRP-NP or NM@MRP-NP ( n = 5). ( H ) Line-scan analysis of the colocalization of Cy5.5 and CD4 + T cells in the immunofluorescence images in Fig. 7D. The statistical analyses were performed as follows: one-way ANOVA followed by Bonferroni’s post hoc comparisons test (F, G). **** indicates p < 0.0001

Article Snippet: The resulting NM@MRP-NP was collected by centrifugation at 20,000×g at 4 °C for 20 min. For the fluorescence tracing experiment, Cy5.5 (MCE, #HY-D0924) was substituted for AZD1208 and encapsulated into the nanoparticles.

Techniques: In Vivo, Fluorescence, Labeling, Immunofluorescence